Polymerase chain reaction (PCR) is a fundamental molecular biology tool that scientists use to amplify and analyze genetic material, such as DNA and RNA. PCR involves the enzymatic amplification of ...

Despite its utility, PCR’s main limitation is that it requires precise cycles of heating and cooling to amplify DNA. The thermal cyclers that perform this operation are clunky, relatively expensive, ...

What is multiple cross displacement amplification? Multiple cross displacement amplification (MCDA) method uses random primers and DNA polymerase to amplify the circular DNA template. The DNA can be ...

The polymerase chain reaction -- A brief comparison between in vivo DNA replication and in vitro PCR amplification -- The PCR in practice -- The different types and varieties of nucleic acid target ...

Limited sample material and insufficient DNA input pose significant challenges for downstream analysis in various laboratory settings. To overcome this issue, isothermal amplification techniques have ...

A new form of PCR has been used to improve the library preparation for forensic DNA typing from difficult samples like touch evidence and human remains. Typically, DNA sequencing and analysis is ...

A DNA strand (purple) primes exponential amplification of DNA (red) as signals for directing light emission of DNA nanodeveices. [Organic and Biomolecular Chemistry] Scientists in Japan say they have ...

Taq polymerase is the heat-stable (thermostable) DNA polymerase extracted from the thermophilic bacteria Thermus aquaticus. Its predominant function is in the polymerase chain reaction (PCR) technique ...

Scientists have developed a way of amplifying DNA on a scale suitable for use in the emerging fields of DNA-based computing and molecular robotics. By enabling highly sensitive nucleic acid detection, ...

Limited sample material resulting in insufficient DNA input is a common hurdle for downstream analysis. This problem can be solved using a variety of methods, including techniques that utilize ...