Steps for deep-tissue 3D-imaging. Embryos or neonates fixed with 4% PFA were optically cleared with 60% TDE or tissue-clearing reagent CUBIC-L/R, and imaged using stereoscopic, confocal, or two-photon ...
Tissue optical clearing is a fundamental technique that enhances light penetration deep into biological samples by reducing light scattering through refractive index homogenization. This process ...
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