We detected the exon 18 mutation using standard PCR and RFLP methods (available on request). We used the Woolf method 3 to test for association in the combined data set and MAPMAKER/SIBS (ref. 4) to ...
1| In a sterile 0.5-ml microcentrifuge tube or amplification tube (on ice), mix the following reagents for the first amplification reaction: 2| Place the tubes in a thermocycler and amplify the ...